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Biopharmaceutics(Medical and Health Sciences)

Tsukamoto TomohitoSpecially Appointed Assistant Professor

2021-2022: JSPS Research Fellowships for Young Scientists (DC2)
2022 March: Ph.D. Graduate school of Pharmaceutical Sciences, Osaka University
2022 December~: Specially Appointed Assistant Professor, Graduate School of Pharmaceutical Sciences, Osaka University

Research theme

Research for the realisation of gene therapy using genome editing technology.

Genome editing technology via CRISPR-Cas9 is expected to be a promising therapeutic approach for congenital genetic diseases. However, there are still several barriers to overcome for the realisation of gene therapy using genome editing, including the inadequate genome editing efficiency of Cas9, the problem of how to deliver Cas9 to target tissues, and immune responses against Cas9.I am collecting basic knowledge to solve the above issues and conducting research on solutions to these issues by making full use of various viral vectors carrying Cas9.

Representative achievements

Sakurai F #, Tsukamoto T #, Ono R, et al. Transduction Properties of an Adenovirus Vector Containing Sequences Complementary to a Liver-Specific microRNA, miR-122a, in the 3'-Untranslated Region of the E4 Gene in Human Hepatocytes from Chimeric Mice with Humanized Liver. #These authors contributed equally to this work. Biol Pharm Bull. 2021;44(10):1506-1513.

Tsukamoto T, Sakai E, Nishimae F, Sakurai F, Mizuguchi H. Efficient generation of adenovirus vectors carrying the Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR associated proteins (Cas)12a system by suppressing Cas12a expression in packaging cells. J Biotechnol. 2019 Oct 10;304:1-9.

Tsukamoto T, Sakai E, Iizuka S, Taracena-Gándara M, Sakurai F, Mizuguchi H. Generation of the Adenovirus Vector-Mediated CRISPR/Cpf1 System and the Application for Primary Human Hepatocytes Prepared from Humanized Mice with Chimeric Liver. Biol Pharm Bull. 2018;41(7):1089-1095.