Graduate School and School of Pharmaceutical Sciences, Osaka University

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Strucure and Function Analysis of Biomolecules（Chemistry）

Fukuda YohtaAssistant professor

2014. Department of Applied Chemistry, Graduate School of Engineering, Osaka University. PhD (Engineering)

Research theme

Precise structural analysis of bio-macromolecules contributing to quantum chemical approaches to biological phenomena

Life is driven by chemical reactions. Microscopic phenomena such as the movement of atoms/electrons in chemical reactions and changes in chemical states of atoms can be described by quantum mechanics. The purpose of my study is experimentally obtaining high-resolution structural information that reflects the positions of hydrogen atoms and the electronic state of each atom in bio-macromolecules. Based on this information, I hope to contribute to the development of quantum-level descriptions of life phenomena.

Exploration of stress tolerance systems ubiquitously found among organisms and elucidation of their mechanisms at the molecular level

All living organisms are constantly exposed to environmental stresses on their cells. By focusing on various organisms that are highly tolerant to environmental stresses, I am searching for ubiquitous stress tolerance systems and elucidating their mechanisms in detail especially at the molecular level, with the aim of applying these systems to the protection of human cells and organs.

Representative achievements

Structural insights into a C2 domain protein specifically found in tardigrades
Yohta Fukuda, Tsuyoshi Inoue
Protein Science 30(2) 513-518 2021

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High-resolution neutron crystallography visualizes an OH-bound resting state of a copper-containing nitrite reductase
Yohta Fukuda, Yu Hirano, Katsuhiro Kusaka, Tsuyoshi Inoue, Taro Tamada
Proceedings of the National Academy of Sciences 117(8) 4071-4077 2020

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Crystal structure of Kumaglobin: a hexacoordinated heme protein from an anhydrobiotic tardigrade, Ramazzottius varieornatus
JeeEun Kim, Yohta Fukuda, Tsuyoshi Inoue
The FEBS Journal 286(7) 1287-1304 2019

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Structural insights into a secretory abundant heat-soluble protein from an anhydrobiotic tardigrade, Ramazzottius varieornatus
Yohta Fukuda, Yoshimasa Miura, Eiichi Mizohata, Tsuyoshi Inoue
FEBS Letters 591(16) 2458-2469 2017

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Redox-coupled proton transfer mechanism in nitrite reductase revealed by femtosecond crystallography
Yohta Fukuda, Ka Man Tsea, Takanori Nakane, Toru Nakatsu, Mamoru Suzuki, Michihiro Sugahara, Shigeyuki Inoue, Tetsuya Masuda, Fumiaki Yumoto, Naohiro Matsugaki, Eriko Nango, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Changyong Song, Takaki Hatsui, Makina Yabashi, Osamu Nureki, Michael E. P. Murphy, Tsuyoshi Inoue, So Iwata, Eiichi Mizohata
Proceedings of the National Academy of Sciences 113(11) 2928-2933 2016

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High-temperature and high-resolution crystallography of thermostable copper nitrite reductase
Yohta Fukuda, Tsuyoshi Inoue
Chemical Communications 51(30) 6532-6535 2015

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