Research
Faculty Members
Quantitative biophysical life science（Chemistry）
Shiraishi YutaroSpecially Appointed Assosiate Professor / Lecturer
2011 B. Pharm (Pharmaceutical Sciences), Faculty of Pharmaceutical Science, The University of Tokyo
2016 Ph.D. (Pharmaceutical Science), Graduate School of Pharmaceutical Science, The University of Tokyo
2016-2017 Research Fellow (PD), Japan Society for the Promotion of Science
2017-2018 Researcher, Japan Biological Informatics Consortium
2018-2019 Researcher, The University of Tokyo
2019-2020 Researcher, Japan Biological Informatics Consortium
2020-2024 Research Scientist, RIKEN Center for Biosystems Dynamics Research
2024-present Specially appointed Lecturer, Graduate School/School of Pharmaceutical Sciences, Osaka University
Research theme
Elucidation of functional mechanism of drug-target proteins
Proteins are inherently dynamic entities, and their dynamic behavior is important as they change conformation upon forming complexes with ligands and effector molecules. We utilize nuclear magnetic resonance (NMR) spectroscopy and other biophysical methods to clarify the functional mechanisms of drug-target proteins based on their dynamic structures.
Representative achievements
Shiraishi Y*, Shimada I*. “NMR Characterization of the Papain-like Protease from SARS-CoV-2 Identifies the Conformational Heterogeneity in Its Inhibitor-Binding Site” J. Am. Chem. Soc. (2023) 145, 30, 16669-16677
Shiraishi Y, Kofuku Y, Ueda T, Pandey S, Dwivedi-Agnihotri H, Shukla AK, Shimada I*. “Biphasic actiovation of β-arrestin 1 upon interaction with a GPCR revealed by methyl-TROSY NMR” Nat. Commun. (2021) 12, 7158
Mizumura T, Kondo K, Kurita M, Kofuku Y, Natsume M, Imai S, Shiraishi Y, Ueda T, Shimada I*. “Activation of adenosine A2A receptor by lipids from docosahexaenoic acid revealed by NMR” Sci. Adv. (2020) 6, eaay8544
Imai S, Yokomizo T, Kofuku Y, Shiraishi Y, Ueda T, Shimada I*. “Structural equilibrium underlying ligand-dependent activation of β2-adrenoceptor” Nat. Chem.Biol. (2020) 16, 430-439
Kofuku Y, Yokomizo T, Imai S, Shiraishi Y, Natsume M, Itoh H, Inoue M, Nakata K, Igarashi S, Yamaguchi H, Mizukoshi T, Suzuki EI, Ueda T, Shimada I*. “Deuteration and selective labeling of alanine methyl groups of β2-adrenergic receptor expressed in a baculovirus-insect cell expression system” J Biomol NMR (2018) 71, 185-192
Shiraishi Y, Natsume M, Kofuku Y, Imai S, Nakata K, Mizukoshi T, Ueda T, Iwaï H, Shimada I*. “Phosphorylation-induced conformation of β2-adrenoceptor related to arrestin recruitment revealed by NMR” Nat. Commun. (2018) 9, 194
Okude J, Ueda T, Kofuku Y, Sato M, Nobuyama N, Kondo K, Shiraishi Y, Mizumura T, Onishi K, Natsume M, Maeda M, Tsujishita H, Kuranaga T, Inoue M, Shimada I*. “Identification of a Conformational Equilibrium That Determines the Efficacy and Functional Selectivity of the μ-Opioid Receptor” Angew. Chem. Int. Ed. Engl. (2015) 54, 15771-15776
Kofuku Y, Ueda T, Okude J, Shiraishi Y, Kondo K, Mizumura T, Suzuki S, Shimada I*. “Functional dynamics of deuterated β2-adrenergic receptor in lipid bilayers revealed by NMR spectroscopy” Angew. Chem. Int. Ed. Engl. (2014) 53, 13376-13379
Kofuku Y, Ueda T, Okude J, Shiraishi Y, Kondo K, Maeda M, Tsujishita H, Shimada I*. “Efficacy of the β₂-adrenergic receptor is determined by conformational equilibrium in the transmembrane region” Nat. Commun. (2012) 3, 1045
