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2023

■論文

Post-Synthetic Nucleobase Modification of DNA by Sonogashira Coupling and Influence of Alkynyl Modifications on the Duplex-Forming Ability
A. Mikami, S. Mori, T. Osawa, S. Obika
Chem. Eur. J.

Applicability of supercritical fluid chromatography for oligonucleotide analysis: A proof-of-concept study
M. Hayashida, R. Suzuki, S. Horie, J. Masuda, T. Yamaguchi, S. Obika
J. Chromatogr. A, 1708, 464333.

Mechanism of the extremely high duplex-forming ability of oligonucleotides modified with N-tert-butylguanidine- or N-tert-butyl-N′-methylguanidine-bridged nucleic acids
T. Yamaguchi, N. Horie, H. Aoyama, S. Kumagai, S. Obika
Nucleic Acids Res., 51, 7749-7761.

Development of 8–17 XNAzymes that are functional in cells
K. Chiba, T. Yamaguchi, S. Obika
Chem. Sci., 14, 7620–7629.

核酸医薬の不純物分析における課題について
高嶺竜太朗、山口卓男
J. Mass Spectrom. Soc. Jpn.（質量分析学会誌）71, 51–54.

An Antisense Amide-bridged Nucleic Acid Gapmer Oligonucleotide Targeting SRRM4 Alters REST Splicing and Exhibits Anti-tumor Effects in Small Cell Lung Cancer and Prostate Cancer Cells
M. Yoshida, C. Oda, K. Mishima, I. Tsuji, S. Obika, M. Shimojo
Cancer Cell Int., 23, 8.

Synthesis of multivalent fatty acid-conjugated antisense oligonucleotide: physical properties, cell internalization, and in vitro and in vivo activities
Y. Tanaka, Y. Tanioku, T. Nakayama, K. Aso, T. Yamaguchi, H. Kamada, S. Obika
Bioorg. Med. Chem., 2023, 81, 117192.

Retention behavior of short double-stranded oligonucleotide and its potential impurities by anion-exchange chromatography under non-denaturing conditions
H. Togawa, T. Okubo, Y. Nonaka, T. Yamaguchi, S. Obika
J. Chromatogr. A, 2023, 1691, 463808.

Synthesis and biophysical properties of tetravalent PEG-conjugated antisense oligonucleotide
T. R. Chowdhury, T. Taufiq , K. Ishiida, M. A. Islam, Y. Kasahara, T. Osawa, S. Obika
Bioorg. Med. Chem., 2023, 78, 117149.

Synthesis and Properties of Nucleobase-Sugar Dual Modified Nucleic Acids: 2-OMe-RNA and scpBNA Bearing a 5-Hydroxycytosine Nucleobase
Y. Sakurai, T. Yamaguchi, T. Yoshida, M. Horiba, T. Inoue, S. Obika
J. Org. Chem., 2023, 88, 154–162.

2’-N-Alkylaminocarbonyl-2’-amino-LNA: Synthesis, Duplex Stability, Nuclease Resistance, and In Vitro Anti-MicroRNA Activity
T. Takegawa-Araki, K. Yasukawa, N. Iwazaki, H. Furukawa, H. Sawamoto, S. Obika
Bioorg. Med. Chem., 78, 117148.

■著書

B．核酸医療の基礎知識　アンチセンス核酸の基礎ー構造と作用機序
山口卓男
Clinical Neuroscience 41巻5号, 41(5), 612–615.

核酸医薬の製造・精製・分析　－いま求められること，そして，将来求められること－
山口卓男
SCAS NEWS, 2023-I, 3–6.

2022

■論文

Development of phosphoramidite reagents for the synthesis of base-labile oligonucleotides modified with a linear aminoalkyl and amino-PEG linker at the 3′-end
T. Osawa, Q. Ren, S. Obika
Molecules, 2022, 27, 8501.

Structure-activity Relationships of Anti-microRNA Oligonucleotides Containing Cationic Guanidine-Modified Nucleic Acids
T. Takegawa-Araki, S. Kumagai, K. Yasukawa, M. Kuroda, T. Sasaki, S. Obika
J. Med. Chem., 2022, 65, 2139–2148.

Synthesis and physical and biological properties of 1,3-diaza-2-oxophenoxazine-conjugated oligonucleotides
R. Yamaji, O. Nakagawa, Y. Kishimoto, A. Fujii, T. Matsumura, T. Nakayama, H. Kamada, T. Osawa, T. Yamaguchi, S. Obika
Bioorg. Med. Chem., 2022, 72, 116972.

Synthesis, duplex-forming ability, and nuclease resistance of oligonucleotides containing a thymidine derivative with a 1-oxaspiro[4.5]decane skeleton
T. Osawa, N. Yano, H. Aoyama, S. Obika
Chem. Pharm. Bull., 2022, 70(10), 699–706.

Conjugation of oligonucleotides with activated carbamate reagents prepared by the Ugi reaction for oligonucleotide library synthesis
R. Kita, T. Osawa, S. Obika
RSC Chem. Biol., 2022, 3(6), 728–738.

Synthesis of Sugar and Nucleoside Analogs and Evaluation of Their Anticancer and Analgesic Potentials
F. Hussain, F. I. Rahman, P. Saha, A. Mikami, T. Osawa, S. Obika and S. M. A. Rahman
Molecules, 2022, 27(11), 3499.

Reduction of Off-Target Effects of Gapmer Antisense Oligonucleotides by Oligonucleotide Extension
H. Yasuhara, T. Yoshida, K. Sasaki, S. Obika, T. Inoue
Mol. Diagn. Ther., 2022, 1, 117.

Physicochemical property evaluation of modification oligonucleotides by traveling-wave ion mobility mass spectrometry
S. Omuro, T. Yamaguchi, T. Kawase, M. Terasaki, K. Hirose, S. Obika
Rapid Commun. Mass Spectrom., 2022, 36, e9279.

■著書

4.3　核酸の分析
大澤昂志、小比賀聡
医薬品開発のための分光法, 2022, 197–206.