大阪大学大学院 薬学研究科
生物有機化学分野チャンネル
→2018   →2017   →バックナンバー
2018
■論文
Construction of a tri-chromatic reporter cell line for the rapid and simple screening of splice-switching oligonucleotides targeting DMD exon 51 using high content screening
T. Shimo, K. Tachibana, S. Obika
PLoS ONE 2018;13(5):e0197373

Generation of splice switching oligonucleotides targeting the Cockayne syndrome group B gene product in order to change the diseased cell state
Y. Sin, F. Makimura, M. Saijo, S. Obika
Biochem. Biophys. Res. Commun. 2018;500(2):163-169

Cholesterol-GalNAc Dual Conjugation Strategy for Reducing Renal Distribution of Antisense Oligonucleotides
F. Wada, T. Yamamoto, T. Ueda, M. Sawamura, S. Wada, M. Harada-Shiba, S. Obika
Nucleic Acid Ther. 2018;28:50-7

Hydrogen peroxide-triggered gene silencing in mammalian cells through boronated antisense oligonucleotides
S. Mori, K. Morihiro, T. Okuda, Y. Kasahara, S. Obika
Chem. Sci. 2018;9:1112-8

A Novel Human Muscle Cell Model of Duchenne Muscular Dystrophy Created by CRISPR/Cas9 and Evaluation of Antisense-Mediated Exon Skipping
T. Shimo, K. Hosoki, Y. Nakatsuji, T. Yokota, S. Obika
J. Hum. Genet. 2018;63:365-75

2017
■論文
Synthesis and thermal stabilities of oligonucleotides containing 2’-O,4’-C-methylene bridged nucleic acid with a phenoxazine base.
Y. Kishimoto, A. Fujii, O. Nakagawa, T. Nagata, T. Yokota, Y. Hari, S. Obika
Org. Biomol. Chem. 2017;38(15):8145-52.

Small Nucleolar Noncoding RNA SNORA23, Upregulated in Human Pancreaic Ductal Adenocarcinoma, Regulates Expression of SYNE2 to Promote Growth and Metastasis of Xenograft Tumors in Mice.
L. Cui, K. Nakano, S. Obchoei, K. Setoguchi, M. Matsumoto, T. Yamamoto, S. Obika, K. Shimada, N. Hiraoka.
Gastroenterogy 2017 July;153:292-306.

Synthesis, duplex-forming ability, enzymatic stability, and in vitro antisense potency of oligonuclreotides including 2’-C, 4’-C-ethyleneoxy-bridged thymedine derivatives.
T. Osawa, M. Sawamura, F. Wada, T. Yamamoto, S. Obika, Y.Hari.
Org. Biomol. Chem. 2017 May;15(18):3955-63.

Synthesis of the 5-Methyluridine Monomer of 3’-O,4’-C-Ethyleneoxy-bridged Nucleic Acid.
T. Osawa, M. Dohi, Y. Hitomi, Y. Ito, S. Obika, Y. Hari.
J Heterocycles. 2017;95(1):342-52.

Phospholamban Inhibition by a Single Dose of Locked Nucleic Acid Antisense Oligonucleotide Improves Cardiac Contractility in Pressure Overload-Induced Systolic Dysfunction in Mice.
H. Morihara, T, Yamamoto, H. Oiwa, K. Tonegawa, D. Tsuchiyama, I. Kawakatsu, M. Obana, M. Maeda, T. Mohri, S. Obika, Y Fujio, H. Nakayama.
J. Cardiovasc. Pharmacol. Ther. 2017 May;22(3):273-82.

Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides.
A. Shimoyama, A. Fujisaka, S. Obika.
J. Pharm. Biomed. Anal. 2017 Mar. 20;136:55-65.

Synthesis and Properties of 7-Deazapurine- and 8-Aza-7-deazapurine-Locked Nucleic Acid Analogues: Effect of the Glycosidic Torsion Angle.
T. Hara, T. Kodama, Y. Takegaki, K. Morihiro, K.R. Ito, S. Obika.
J. Org. Chem. 2017;82(1):25-36.

Triazole- and Tetrazole-Bridged Nucleic Acids: Synthesis, Duplex Stability, Nuclease Resistance, and in Vitro and in Vivo Antisense Potency.
Y. Mitsuoka, T. Yamamoto, A. Kugimiya, R. Waki, F. Wada, S. Tahara, M. Sawamura, M. Noda, Fujimura Y, Y. Kato, Y. Hari, S. Obika.
J Org. Chem. 2017 Jan. 6;82(1):12-24.

■総説
Designing Effective Antisense Oligonucleotides for Exon Skipping.
T. Shimo, R. Maruyama, T. Yokota.
Methods Mol Biol. 2017;1687:143-55.

Biological applications of xeno nucleic acids.
K. Morihiro, Y. Kasahara, S. Obika.
Mol. Biosyst. 2017 Jan. 31;13(2):235-45.

核酸医薬開発における化学
小比賀 聡、笠原 勇矢
日本発次世代創薬のための化学, 2017, 70-1, 020-2, 化学と工業

大阪大学大学院 薬学研究科 生物有機化学分野
〒565‐0871 大阪府吹田市山田丘1-6
TEL: 06-6879-8200    FAX: 06-6879-8204
obika@phs.osaka-u.ac.jp