大阪大学大学院 薬学研究科
生物有機化学分野チャンネル
→2024   →2023   →バックナンバー
2024
■論文
Synthesis of coumarin-conjugated oligonucleotides via Knoevenagel condensation to prepare an oligonucleotide library
T. Osawa, S. Obika
Chem. Pharm. Bull., 72(2), 143–148.

Synthesis, duplex-forming ability, and enzymatic stability of oligonucleotides modified with amide-linked dinucleotides containing a 3′,4′-tetrahydropyran-bridged nucleic acid
T. Osawa, T. Akino, S. Obika
J. Org. Chem. 89(1), 269–280.

Development of a simple purification method for oligonucleotides synthesized by using a phosphoramidite for 5'-end modification as a capping reagent
Q. Ren, T. Osawa, S. Obika
Tetrahedron, 150, 133774.

2023
■論文
Systematic Analysis of 2′-O-Alkyl Modified Analogs for Enzymatic Synthesis and Their Oligonucleotide Properties
K. Ishida, Y. Kasahara, H. Hoshino, T. Okuda, S. Obika
Molecules, 28 (23), 7911.

強塩基処理できないオリゴ核酸の3'-アミノリンカー修飾法の開発
大澤昂志
日本核酸化学会誌, 7, 3–8.

複数種の液体クロマトグラフ質量分析計を用いたモデル核酸医薬品の分析データの比較
廣瀬賢治,吉田徳幸,寺崎真樹,瀬崎浩史,唐澤 薫,岩崎了教,高原健太郎,滝口直美,関口光明,南海浩一,斎藤恵美,佐藤秀昭,大澤昂志,山口卓男,伊藤浩介,川上純司,小比賀聡,井上貴雄
医薬品医療機器レギュラトリーサイエンス , 54(5), 439–454.

Post-Synthetic Nucleobase Modification of DNA by Sonogashira Coupling and Influence of Alkynyl Modifications on the Duplex-Forming Ability
A. Mikami, S. Mori, T. Osawa, S. Obika
Chem. Eur. J.

Applicability of supercritical fluid chromatography for oligonucleotide analysis: A proof-of-concept study
M. Hayashida, R. Suzuki, S. Horie, J. Masuda, T. Yamaguchi, S. Obika
J. Chromatogr. A, 1708, 464333.

Mechanism of the extremely high duplex-forming ability of oligonucleotides modified with N-tert-butylguanidine- or N-tert-butyl-N′-methylguanidine-bridged nucleic acids
T. Yamaguchi, N. Horie, H. Aoyama, S. Kumagai, S. Obika
Nucleic Acids Res., 51, 7749-7761.

Novel Strategy of Liver Cancer Treatment with Modified Antisense Oligonucleotides Targeting Human Vasohibin-2
S. Horie, Y. Suzuki, T. Yamamoto, S. Obika, K. Mohri, C. Kiyota, Q. Ren, S. Warashina, Y. Wada, Y. Watanabe, H. Mukai, Y. Sato
Cancer Sci., 114, 3740–3749.

Development of 8–17 XNAzymes that are functional in cells
K. Chiba, T. Yamaguchi, S. Obika
Chem. Sci., 14, 7620–7629.

Inhibition of MicroRNA-33b in Humanized Mice Ameliorates Nonalcoholic Steatohepatitis
S. Miyagawa, T. Horie, T. Nishino, S. Koyama, T. Watanabe, O. Baba, T. Yamasaki, N. Sowa, C. Otani, K. Matsushita, H. Kojima, M. Kimura, Y. Nakashima, S. Obika, Y. Kasahara, J. Kotera, K. Oka, R. Fujita, T. Sasaki, A. Takemiya, K. Hasegawa, T. Kimura, K. Ono
Life Science Alliance, 6, e202301902.

核酸医薬の不純物分析における課題について
高嶺竜太朗、山口卓男
J. Mass Spectrom. Soc. Jpn.(質量分析学会誌)71, 51–54.

Synthesis and Properties of A Novel Modified Nucleic Acid, 2′-N-Methanesulfonyl-2′-Amino-locked Nucleic Acid
H. Sawamoto, T. Sasaki, T. Takegawa-Araki, M. Utsugi, H. Furukawa, Y. Hirakawa, F. Yamairi, T. Kurita, K. Murahashi, K. Yamada, T. Ohta, S. Kumagai, A. Takemiya, S. Obika, J. Kotera
Bioorg. Med. Chem. Lett., 88, 129289.

An Antisense Amide-bridged Nucleic Acid Gapmer Oligonucleotide Targeting SRRM4 Alters REST Splicing and Exhibits Anti-tumor Effects in Small Cell Lung Cancer and Prostate Cancer Cells
M. Yoshida, C. Oda, K. Mishima, I. Tsuji, S. Obika, M. Shimojo
Cancer Cell Int., 23, 8.

Synthesis of multivalent fatty acid-conjugated antisense oligonucleotide: physical properties, cell internalization, and in vitro and in vivo activities
Y. Tanaka, Y. Tanioku, T. Nakayama, K. Aso, T. Yamaguchi, H. Kamada, S. Obika
Bioorg. Med. Chem., 2023, 81, 117192.

Retention behavior of short double-stranded oligonucleotide and its potential impurities by anion-exchange chromatography under non-denaturing conditions
H. Togawa, T. Okubo, Y. Nonaka, T. Yamaguchi, S. Obika
J. Chromatogr. A, 2023, 1691, 463808.

Synthesis and biophysical properties of tetravalent PEG-conjugated antisense oligonucleotide
T. R. Chowdhury, T. Taufiq , K. Ishiida, M. A. Islam, Y. Kasahara, T. Osawa, S. Obika
Bioorg. Med. Chem., 2023, 78, 117149.

Synthesis and Properties of Nucleobase-Sugar Dual Modified Nucleic Acids: 2-OMe-RNA and scpBNA Bearing a 5-Hydroxycytosine Nucleobase
Y. Sakurai, T. Yamaguchi, T. Yoshida, M. Horiba, T. Inoue, S. Obika
J. Org. Chem., 2023, 88, 154–162.

2’-N-Alkylaminocarbonyl-2’-amino-LNA: Synthesis, Duplex Stability, Nuclease Resistance, and In Vitro Anti-MicroRNA Activity
T. Takegawa-Araki, K. Yasukawa, N. Iwazaki, H. Furukawa, H. Sawamoto, S. Obika
Bioorg. Med. Chem., 78, 117148.

■著書
アンチセンス核酸の創薬戦略
小比賀聡
Medical Science Digest, 49(14), 3(733)–5(735).

核酸医薬開発における化学の重要性
山口卓男
Medical Science Digest, 49(14), 6(736)–9(739).

LC/MSを使用したオリゴ核酸の不純物分析
瀬崎浩史、内藤厚子、林明生、川上純司、井上貴雄、山口卓男、小比賀聡
Medical Science Digest, 49(14), 50(780)–52(782).

B.核酸医療の基礎知識 アンチセンス核酸の基礎ー構造と作用機序
山口卓男
Clinical Neuroscience, 41(5), 612–615.

核酸医薬の製造・精製・分析 -いま求められること,そして,将来求められること-
山口卓男
SCAS NEWS, 2023-I, 3–6.
大阪大学大学院 薬学研究科 生物有機化学分野
〒565‐0871 大阪府吹田市山田丘1-6
TEL: 06-6879-8200    FAX: 06-6879-8204
obika@phs.osaka-u.ac.jp